Journal: The Journal of Cell Biology

Article Title: Suppression of Pyk2 Kinase and Cellular Activities by Fip200

doi:

Figure Lengend Snippet: FIP200 inhibition of Pyk2-induced apoptosis. (A) Rat-1 cells were cotransfected with a plasmid encoding GFP and expression vectors encoding Pyk2, FIP200, NT-FIP, CT-FIP, or vector alone (control), as indicated. The results show the mean and SD of a percentage of apoptotic cells among the positively transfected (GFP+) cells from at least four independent experiments. * P < 0.01 and ** P ≥ 0.15 in comparison to the value from cells transfected with the control vector (Minitab Release 10.5 Xtra). (B) Rat-1 cells were cotransfected with a plasmid encoding GFP, pKH3-Pyk2 (P), and expression vectors encoding FIP200, NT-FIP, CT-FIP, or vector alone (C), as indicated. They were analyzed as in A, and the results show the mean and SD of a percentage of apoptotic/GFP positive cells. * P < 0.01 and ** P > 0.40 in comparison to the value from cells transfected with Pyk2 and a control vector (Minitab Release 10.5 Xtra).

Article Snippet: Statistical analyses were performed by Minitab Release 10.5Xtra (Minitab Inc.).

Techniques: Inhibition, Plasmid Preparation, Expressing, Transfection